Sample registration DNA/RNA extraction Library generation Create pool Transfer and import Analysis Reporting

Sample registration

Step 1:

Easily fill in all relevant sample data by using the input mask or import from existing databases.

Structure your research samples by assigning specific projects. Use the build-in tools to manage your project progress.


Step 2:

Choose your desired method and start DNA extraction: the ionLIMS will create extraction plans and provide sample labels for your reaction tubes (optional).

After extraction and DNA concentration measurement (e.g. with QuBit) enter your data and proceed with optional QC-steps (e.g. quantitative real-time PCR / RNAseP). The ionLIMS setup assistent will take care of sample import sheets, pipetting schemes and calculates dilution factors and final results for you.

Library generation

Step 3:

Choose barcode and library kit and start working.

The ionLIMS takes care of the necessary DNA amounts (user adjustable) and creates pipetting schemes for your wet lab procedures. The ionLIMS qPCR assistent finally guides you through all the steps of library QC measurement.

Pool creation

Step 4:

Select all libraries for your sequencing pool. The ionLIMS will check for duplicate barcodes and missing DNA/RNA pairs for combined DNA/fusion analysis.

Just select your desired sequencing chip and chemistry and the ionLIMS will provide you with a sample sheet for direct import to the TorrentSuite software for easy run planning.

Data transfer

Step 5:

Finished sequencing? Then just wait for the TorrentSuite coverage- and variant caller plugins and then start data transfer and import into the ionLIMS. It's that simple!

The ionLIMS will transfer the most important data files (e.g. bam- and vcf-files) to your workdata-folder with intuitiv file renaming. Finally, the ionLIMS optionally annotates your variants. Life can be so easy.

Analysis module

Step 6:

The analysis module helps you with an intuitive user interface, displaying all relevant data for easy sample validation.

Wants to get more out of your data. Try the build-in CNV analysis or BamCounter tool.


Step 7:

Finally automatically generate your sequencing report in Word or PDF format. All customisable in the way you like it.

Variants grouped into different classes will be displayed as tables with all you sample head data, optionally including details about the location of the variant in relation to protein domains.